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p ampk thr 172  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p ampk thr 172
    Alteration of muscle FGF21 and downstream signaling in Ogg1 Tg mice. A , volcano plot of differentially expressed genes (DEGs) in skeletal muscle of Ogg1 Tg mice. Fgf21 is the most upregulated gene among all DEGs. B , Fgf21 gene expression was measured in major tissues of WT and Ogg1 Tg mice using quantitative PCR. Gene expressions were normalized to 18S rRNA expression. C , plasma FGF21was measured in WT and Ogg1 Tg mice using ELISA. D and E , phosphorylation of <t>AMPK</t> and ACC was measured in the skeletal muscle of WT and Ogg1 Tg mice using PAGE, and images were quantified using ImageJ. (n = 3–7); data are expressed as mean ± SD; ∗ p < 0.05 relative to WT. ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein; FGF21, fibroblast growth factor 21; Ogg1 Tg , OGG1-transgenic.
    P Ampk Thr 172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "OGG1 increases exercise endurance via elevated skeletal muscle FGF21"

    Article Title: OGG1 increases exercise endurance via elevated skeletal muscle FGF21

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2026.111360

    Alteration of muscle FGF21 and downstream signaling in Ogg1 Tg mice. A , volcano plot of differentially expressed genes (DEGs) in skeletal muscle of Ogg1 Tg mice. Fgf21 is the most upregulated gene among all DEGs. B , Fgf21 gene expression was measured in major tissues of WT and Ogg1 Tg mice using quantitative PCR. Gene expressions were normalized to 18S rRNA expression. C , plasma FGF21was measured in WT and Ogg1 Tg mice using ELISA. D and E , phosphorylation of AMPK and ACC was measured in the skeletal muscle of WT and Ogg1 Tg mice using PAGE, and images were quantified using ImageJ. (n = 3–7); data are expressed as mean ± SD; ∗ p < 0.05 relative to WT. ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein; FGF21, fibroblast growth factor 21; Ogg1 Tg , OGG1-transgenic.
    Figure Legend Snippet: Alteration of muscle FGF21 and downstream signaling in Ogg1 Tg mice. A , volcano plot of differentially expressed genes (DEGs) in skeletal muscle of Ogg1 Tg mice. Fgf21 is the most upregulated gene among all DEGs. B , Fgf21 gene expression was measured in major tissues of WT and Ogg1 Tg mice using quantitative PCR. Gene expressions were normalized to 18S rRNA expression. C , plasma FGF21was measured in WT and Ogg1 Tg mice using ELISA. D and E , phosphorylation of AMPK and ACC was measured in the skeletal muscle of WT and Ogg1 Tg mice using PAGE, and images were quantified using ImageJ. (n = 3–7); data are expressed as mean ± SD; ∗ p < 0.05 relative to WT. ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein; FGF21, fibroblast growth factor 21; Ogg1 Tg , OGG1-transgenic.

    Techniques Used: Gene Expression, Real-time Polymerase Chain Reaction, Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Phospho-proteomics, Transgenic Assay



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    Alteration of muscle FGF21 and downstream signaling in Ogg1 Tg mice. A , volcano plot of differentially expressed genes (DEGs) in skeletal muscle of Ogg1 Tg mice. Fgf21 is the most upregulated gene among all DEGs. B , Fgf21 gene expression was measured in major tissues of WT and Ogg1 Tg mice using quantitative PCR. Gene expressions were normalized to 18S rRNA expression. C , plasma FGF21was measured in WT and Ogg1 Tg mice using ELISA. D and E , phosphorylation of <t>AMPK</t> and ACC was measured in the skeletal muscle of WT and Ogg1 Tg mice using PAGE, and images were quantified using ImageJ. (n = 3–7); data are expressed as mean ± SD; ∗ p < 0.05 relative to WT. ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein; FGF21, fibroblast growth factor 21; Ogg1 Tg , OGG1-transgenic.
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    Alteration of muscle FGF21 and downstream signaling in Ogg1 Tg mice. A , volcano plot of differentially expressed genes (DEGs) in skeletal muscle of Ogg1 Tg mice. Fgf21 is the most upregulated gene among all DEGs. B , Fgf21 gene expression was measured in major tissues of WT and Ogg1 Tg mice using quantitative PCR. Gene expressions were normalized to 18S rRNA expression. C , plasma FGF21was measured in WT and Ogg1 Tg mice using ELISA. D and E , phosphorylation of <t>AMPK</t> and ACC was measured in the skeletal muscle of WT and Ogg1 Tg mice using PAGE, and images were quantified using ImageJ. (n = 3–7); data are expressed as mean ± SD; ∗ p < 0.05 relative to WT. ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein; FGF21, fibroblast growth factor 21; Ogg1 Tg , OGG1-transgenic.
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    Figure 7. Effect of Ramon flour on hepatic protein abundance of <t>AMPK</t> and P-AMPK (A); HSP70 (B); NRF2 (C), and SOD2 (D) as assessed with GADPH used as the loading control. Data are expressed as the mean ± SEM (n = 5). The data were analyzed using a one-way analysis of variance (ANOVA) test with a post hoc Tukey’s test. Differences between experimental groups are shown with letters, where a > b > c. Statistical differences are considered with a value of p < 0.05. C: control diet (AIN-93); C + RF: control diet adjusted with 25% Ramon flour; HFD: high-fat diet + 5% sugar in water; and HFD + RF: high-fat diet adjusted with 25% Ramon flour + 5% sugar in water.
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    Image Search Results


    Alteration of muscle FGF21 and downstream signaling in Ogg1 Tg mice. A , volcano plot of differentially expressed genes (DEGs) in skeletal muscle of Ogg1 Tg mice. Fgf21 is the most upregulated gene among all DEGs. B , Fgf21 gene expression was measured in major tissues of WT and Ogg1 Tg mice using quantitative PCR. Gene expressions were normalized to 18S rRNA expression. C , plasma FGF21was measured in WT and Ogg1 Tg mice using ELISA. D and E , phosphorylation of AMPK and ACC was measured in the skeletal muscle of WT and Ogg1 Tg mice using PAGE, and images were quantified using ImageJ. (n = 3–7); data are expressed as mean ± SD; ∗ p < 0.05 relative to WT. ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein; FGF21, fibroblast growth factor 21; Ogg1 Tg , OGG1-transgenic.

    Journal: The Journal of Biological Chemistry

    Article Title: OGG1 increases exercise endurance via elevated skeletal muscle FGF21

    doi: 10.1016/j.jbc.2026.111360

    Figure Lengend Snippet: Alteration of muscle FGF21 and downstream signaling in Ogg1 Tg mice. A , volcano plot of differentially expressed genes (DEGs) in skeletal muscle of Ogg1 Tg mice. Fgf21 is the most upregulated gene among all DEGs. B , Fgf21 gene expression was measured in major tissues of WT and Ogg1 Tg mice using quantitative PCR. Gene expressions were normalized to 18S rRNA expression. C , plasma FGF21was measured in WT and Ogg1 Tg mice using ELISA. D and E , phosphorylation of AMPK and ACC was measured in the skeletal muscle of WT and Ogg1 Tg mice using PAGE, and images were quantified using ImageJ. (n = 3–7); data are expressed as mean ± SD; ∗ p < 0.05 relative to WT. ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein; FGF21, fibroblast growth factor 21; Ogg1 Tg , OGG1-transgenic.

    Article Snippet: p-AMPK (Thr-172) , 2535- Cell Signaling Technologies , 1:1000.

    Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Phospho-proteomics, Transgenic Assay

    Figure 7. Effect of Ramon flour on hepatic protein abundance of AMPK and P-AMPK (A); HSP70 (B); NRF2 (C), and SOD2 (D) as assessed with GADPH used as the loading control. Data are expressed as the mean ± SEM (n = 5). The data were analyzed using a one-way analysis of variance (ANOVA) test with a post hoc Tukey’s test. Differences between experimental groups are shown with letters, where a > b > c. Statistical differences are considered with a value of p < 0.05. C: control diet (AIN-93); C + RF: control diet adjusted with 25% Ramon flour; HFD: high-fat diet + 5% sugar in water; and HFD + RF: high-fat diet adjusted with 25% Ramon flour + 5% sugar in water.

    Journal: Antioxidants (Basel, Switzerland)

    Article Title: Ramon Flour ( Brosimum alicastrum Swartz) Ameliorates Hepatic Lipid Accumulation, Induction of AMPK Phosphorylation, and Expression of the Hepatic Antioxidant System in a High-Fat-Diet-Induced Obesity Mouse Model.

    doi: 10.3390/antiox12111957

    Figure Lengend Snippet: Figure 7. Effect of Ramon flour on hepatic protein abundance of AMPK and P-AMPK (A); HSP70 (B); NRF2 (C), and SOD2 (D) as assessed with GADPH used as the loading control. Data are expressed as the mean ± SEM (n = 5). The data were analyzed using a one-way analysis of variance (ANOVA) test with a post hoc Tukey’s test. Differences between experimental groups are shown with letters, where a > b > c. Statistical differences are considered with a value of p < 0.05. C: control diet (AIN-93); C + RF: control diet adjusted with 25% Ramon flour; HFD: high-fat diet + 5% sugar in water; and HFD + RF: high-fat diet adjusted with 25% Ramon flour + 5% sugar in water.

    Article Snippet: Then, the membranes were incubated in blocking solution overnight at 4 ◦C with one of the following primary antibodies: AMPK1/2 (1:1000; sc-, Santa Cruz Biotechnologies, Santa Cruz, CA, USA), P-AMPK (Thr-172) (1:1000; sc-, Santa Cruz Biotechnologies, Santa Cruz, CA, USA), NRF2 (1:500; #12721 Cell Signaling, Danvers, MA, USA), SOD2 (1:3000; 06-984 Merk Millipore Burlington, MA, USA), or HSP70 (1:10,000; SAB4200714 Sigma Aldrich, San Luis, MO, USA).

    Techniques: Quantitative Proteomics, Control